Subcellular western blotting of single cells

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Subcellular western blotting of single cells

Although immunoassays are the de facto standard for determining subcellular protein localization in individual cells, antibody probe cross-reactivity and fixation artifacts remain confounding factors. To enhance selectivity while providing single-cell resolution, we introduce a subcellular western blotting technique capable of separately assaying proteins in the 14 pL cytoplasm and 2 pL nucleus...

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ژورنال

عنوان ژورنال: Microsystems & Nanoengineering

سال: 2017

ISSN: 2055-7434

DOI: 10.1038/micronano.2016.79